Breath sampling could be used for managing and mitigation in future public health challenges, he said.ĭravid said he imagines people entering hotels, hospitals and schools, picking up one of his tests to breathe into, and then knowing whether they’re infected by the time they’re on the other side of the room. Shekhawat said children back in school combined with the well-researched fact that underserved populations are at higher risk of exposure underscores the need for non-invasive and rapid diagnostics approaches. “Because the sensitivity of the technique is so good, breath has a lower viral load, but it has enough virus for this technology to detect.” “Instead of nasal swabs, we want to use breath,” Dravid said. Dravid said they are looking for ways to scale and produce the technology and ultimately bring it to mobile devices, using the approach to bring no-contact testing to the public. In addition to incorporating multiple variants and diseases into the mechanism, the team’s goal is to expand the platform to hold more capabilities. “We also are developing integrated microfluidics that will allow us to dip multiple cantilevers into antibody solutions and detect multiple viral loads at the same time.” “We have some initial data to demonstrate the high sensitivity to other diseases in addition to the coronavirus,” Shekhawat said. ![]() Shekhawat, a co-corresponding author and Northwestern materials science and engineering research professor, said the technology is not limited to COVID-19 detection. If COVID-19 antibodies were present in the sample, they caused the thin cantilever with corresponding antigens to bend, with each finger serving as an extra line of defense for any other antigens. Then, collected swab samples were grafted onto the cantilever surface. The team applied coatings of different COVID-19 antigens onto each “finger” of five silicon cantilevers next to an additional one for reference. After optimizing the old technology for use with electronic imaging, Dravid’s lab worked with scientists at the Northwestern University Feinberg School of Medicine to acquire patient swab samples and use data to understand how diseases move through communities. The tiny cantilevers are made of silicon and can be easily reproduced by following a mold, making them an attractive option for potential mass-production. And unlike most sensors available that rely on just one protein, we can look at multiple targets at the same time.” “Microcantilevers can give you a faster turnaround - within two or three minutes - because they leverage specific affinity surface binding. ![]() “We were excited to apply the technology because these are micro- and nanosystems that don’t need a lot of viral material to make a difference,” Dravid said. The platform, which leverages microscopic cantilevers, was originally developed 15 years ago to detect protein interactions with DNA, so could effectively be “dusted off” and repurposed, according to corresponding author and Northwestern materials science and engineering professor Vinayak P. The final version will be published tomorrow (Oct. Published in the journal Biosensors and Bioelectronics, the paper is titled “Highly sensitive and ultra-rapid antigen-based detection of SARS-CoV-2 using nanomechanical sensor platform.” A pre-proof version of the paper is available online. The high-sensitivity test is also being developed as a rapid exhaled breath COVID-19 detection method, similar to a breathalyzer test. The rapid swab test uses a nanomechanical platform to detect multiple surface proteins on the COVID-19 virus, and shows potential to differentiate among different variants and viruses. ![]() Now, a novel antigen-based COVID-19 detection method developed in a Northwestern University laboratory demonstrated 100% accuracy in a blind test in five or fewer minutes from swab to signal. But to effectively slow the rate of infection, spaces like schools and hospitals need ways to accurately identify infected individuals within their first days of infection as they’re walking in the door. Furthermore, rapid antigen alternatives struggle to match up to the gold standard. New variants to the COVID-19 virus have challenged the capacity of existing testing methods to deliver fast and consistently accurate test results, as PCR tests must be sent to labs and can take days to be returned.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |